Background: The purpose of the study was to find out the effect of training on selected morphological, physiological and biochemical variables in soccer players under 19 years of age. Material/Methods: A total of 30 Indian male soccer players under 19 years of age (age: 16.00–18.99 yr) volunteered for this study. The training sessions were divided into 2 phases (i) Preparatory Phase (PP, 8 weeks) and (ii) Competitive Phase (CP, 4 weeks). The training programme consisted of aerobic, anaerobic and skill development, and was completed 4 hrs/day; 5 days/week. Selected morphological, physiological and biochemical variables were measured at zero level (baseline data, BD) and at the end of PP and CP. Results: A significant increase (P<0.05) in lean body mass, VO2max, anaerobic power, grip and back strength, urea and uric acid levels; and a decrease (P<0.05) in percentage of body fat, haemoglobin, total cholesterol, and LDL-C levels have been noted in PP and CP when compared to BD. However, no significant change was noted in stature, body mass, maximal heart rate, triglyceride and HDL-C levels of the players after the training. Conclusions: This study would provide useful information for training and selection of soccer players of under-19-year-old groups.