Background: The aim of this study was to determine physical activity profiles of the main referee and the assistant referee during official championship matches. Material/Methods: Motor activity of 10 referees and 10 assistant referees was studied during 10 matches of the championship rank in the period from April to November 2016. Using a POLAR M400 heart rate monitor with a built-in GPS sensor, the total distance covered during refereeing, the mean and the maximum speed, and the intensity of the refereeing effort were analyzed. Results: It was found that the main referee covered the mean distance of 7.75 km, while the assistant referee 4.40 km. The main referee’s mean heart rate was higher by 25 bt/min than that of the assistant, and the mean maximum heart rates were 184.9 bt/min and 166.6 bt/min, respectively. The assistant referee had the mean maximum running speed of 19.97 km/h. In general, the mean running speed of the main referee was higher than the assistant referee’s mean speed. Conclusions: Differences in physical activity between the main referee and the assistant referee indicate.